A Vascular Graft On‐a‐Chip Platform for Assessing the Thrombogenicity of Vascular Prosthesis and Coatings with Tuneable Flow and Surface Conditions

Abstract Vascular grafts are essential for the management of cardiovascular disease. However, lifesaving potential these devices is undermined by thrombosis arising from material and flow interactions on blood contacting surface. To combat this issue, antithrombogenic coatings have emerged as a promising strategy modulating graft interaction in vivo. Although an important determinant performance, hemodynamics frequently overlooked vitro testing their translatability remains poorly understood. Herein, limitation addressed with microscale on‐a‐chip platform incorporating vascular prosthesis tuneable surface conditions vitro. As proof concept, used to test thrombogenicity novel class lubricant‐infused (LIS) antibody (anti‐CD34 LIS) coated expanded polytetrafluoroethylene (ePTFE) presence arterial wall shear stress without endothelial cells. The findings suggest LIS ePTFE thromboresistant under significantly reduced fibrin(ogen) deposition, thrombin activity, cell adhesion compared uncoated controls. It moreover apparent that properties device advantageous translation blood‐contacting materials general.